Cell-free coupling of influenza virus RNA transcription and translation

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Transcription and replication of influenza virus RNA.

The Influenza viruses are among the most dangerous human pathogens, since they have a wide animal reservoir and re-emerge continuously in the human population. They constitute a collection of highly variable pathogens that cause respiratory infections in man. Influenza type A viruses contain a segmented genome composed by 8 RNA molecules of negative polarity. Each one replicates and transcribe ...

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Coupling between genome translation and replication in an RNA virus.

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Coupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA ( vesicular stomatitis virus ribonucleoprotein cores / virion - associated RNA polymerase / cell - free protein synthesis / slab gel electrophoresis )

The virion transcriptase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of vesicular stomatitis virus was fully active when ribonucleoprotein cores from purified virions were added to cell-free protein synthesizing systems of eukaryotic origin. Synthesis of mRNA was linear for at least 3 hr and the newly synthesized viral mRNA was efficiently utilized for the synthesis of viral...

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Transcription of a recombinant influenza virus RNA in cells that can express the influenza virus RNA polymerase and nucleoprotein genes.

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1977

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.22.2.247-255.1977